Figure 3. Ces1d hydrolyzes lipid droplets isolated from adipose tissue of WT mice and produce unique free fatty acids (FFAs).

(A) TLC of the products of the neutral lipids hydrolyzed by His-Atgl or His-Ces1d. The FFAs are circulated in the panel. The neutral lipids isolated from adipose tissue of WT mice were mixed with His-Atgl or His-Ces1d proteins that were purified from 293T cells. The mixtures were rotated at 37°C for 1 h. STD, standards of lipids; TAG, triacylglycerol; FFA, free fatty acids; DAG, diacylglycerol; MAG, monoacylglycerol. Loadings were normalized to the same quantity of biomass. (A, B, C, D, E) The categories of the FFAs that were identified by LC-MS/MS from the fatty acid fractions as indicated by ellipse in (A). The intensities of each fatty acid were normalized to the control group.

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