(A) TLC of the products of the neutral lipids hydrolyzed by His-Atgl or His-Ces1d. The FFAs are circulated in the panel. The neutral lipids isolated from adipose tissue of WT mice were mixed with His-Atgl or His-Ces1d proteins that were purified from 293T cells. The mixtures were rotated at 37°C for 1 h. STD, standards of lipids; TAG, triacylglycerol; FFA, free fatty acids; DAG, diacylglycerol; MAG, monoacylglycerol. Loadings were normalized to the same quantity of biomass. (A, B, C, D, E) The categories of the FFAs that were identified by LC-MS/MS from the fatty acid fractions as indicated by ellipse in (A). The intensities of each fatty acid were normalized to the control group.
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