Figure 6. Deficiency of Ces1d in adipose tissue affects the dynamics of lipid droplets.

(A) WB analysis of Perilipins (PLINs) including PLIN1, PLIN2, PLIN3, and PLIN5 in the lysates from the sWAT of WT and FKO mice fed on HFD. β-Actin was used as loading control (n = 3 per group; representative of three repeats). (A, B) Quantification of the band intensity in (A) (n = 3 per group, each point represents a biology replicate). Data are represented as mean ± SEM, t test, *P < 0.05. (C) WB analysis of PLIN1, PLIN2, PLIN3, and PLIN5 in the lysates from the liver of WT and FKO mice fed on HFD. GAPDH was used as loading control (n = 4 per group, representative of three repeats). (C, D) Quantification of the band intensity in (C) (n = 4 per group, each point represents a biology replicate). Data are represented as mean ± SEM, t test, *P < 0.05. (E) WB analysis of PLIN1, PLIN2, PLIN3, and PLIN5 in the lysates from the BAT of WT and FKO mice fed on HFD. GAPDH was used as loading control. (n = 5 per group, representative of three repeats). (E, F) Quantification of the band intensity in (E) (n = 5 per group, each point represents a biology replicate). Data are represented as mean ± SEM, t test, *P < 0.05. (G) qPCR analysis for the mRNA levels of de novo lipogenesis related genes including Acaca, Fasn, and Scd1 in the sWAT from WT and FKO mice fed on regular chow or HFD (n = 5 in each group, each point represents a biology replicate, representative of three repeats). Data are represented as mean ± SEM, one-way ANOVA. (H) WB analysis of pACC1, ACC1, pAMPK, and AMPK in the lysates from the BAT of WT and FKO mice fed on HFD. β-Actin was used as the loading control (n = 4 per groups, representative of three repeats). (H, I, J) Quantification of the band intensity for pACC1/ACC1 ratio (I) and pAMPK/AMPK ratio (J) in (H) by ImageJ software (n = 4 per group, each point represents a biology replicate). Data are represented as mean ± SEM, t test, *P < 0.05, ***P < 0.001.