Figure S1. Comparison of the parameters of lipid metabolism and energy expenditure between the WT and FKO mice.

(A, B, C, D, E, F) The levels of TAG (A), FFA (B), glycerol (C), cholesterol (D), HDL (E), and LDL/VLDL (F) in the plasma of WT and FKO mice fed on regular chow (n = 8 in WT group and n = 5 in FKO group, each point represents a biology replicate, representative of three repeats). Data are represented as mean ± SEM, t test. (G) qPCR analysis for the mRNA levels of lipases and related genes including Pnpla2, Lipe, Mgll, and Plin1 in the BAT of WT and FKO mice fed on regular chow or HFD (n = 5 per group, each point represents a biology replicate, representative of three repeats). Data are represented as mean ± SEM, one-way ANOVA, *P < 0.05. (H) qPCR analysis for the mRNA levels of lipases including Pnpla2, Lipe, and Mgll in the liver from WT and FKO mice fed on regular chow or HFD (n = 5 per group, each point represents a biology replicate, representative of three repeats). Data are represented as mean ± SEM, one-way ANOVA, *P < 0.05, **P < 0.01. (I) qPCR analysis for the mRNA levels of De novo lipogenesis genes including Fasn, Acaca, Acacb, and Scd1 in the liver from WT and FKO mice fed on regular chow or HFD (n = 5 per group, each point represents a biology replicate, representative of three repeats). Data are represented as mean ± SEM, one-way ANOVA. (J, K, L, M, N) Metabolic cage studies by indirect calorimetric measurements on the energy expenditure of the WT and FKO mice under HFD feeding. (n = 5 per group). Data are represented as mean ± SEM, one-way ANOVA. (O, P, Q, R, S) Metabolic cage studies indirect calorimetric measurements on the energy expenditure of the WT and FKO mice under regular chow feeding. (n = 5 per group). Data are represented as mean ± SEM, one-way ANOVA.