Figure 2.

Purinosomes display liquid-like properties.A, a purinosome-positive HeLa cell was imaged by LLSM, and the sphericity of purinosomes was observed (white ROI) and (B) calculated across 41 purinosomes. Additional ROIs (magenta and yellow) are presented in Fig. S2A. C, time-lapse imaging of a purinosome-positive HeLa cell showing (D) the coalescing of a purinosome (1) with another (2, arrow) between 360 and 375 s. E, trajectory of both purinosomes over the entire time-lapse experiment (500 s). F, the area of the individual purinosome 1 (0.045 μm², orange line) and 2 (0.132 μm², blue line) before (320–360 s) and after merging (370–415 s). Area of the coalesced purinosome was 0.182 μm² (black line). Solid lines represent average purinosome area across the time points observed. LLSM, lattice light sheet microscopy; ROI, region of interest.