|
Single-cell cultivation
|
| W/O/W |
E. coli
|
Cell culture (from proliferation to death)56
|
| W/O/W |
Oral microbiota of Siberian bear |
Cell culture (with antibiotics)31
|
| Human fecal microbiota from patient and healthy donors |
| W/O/W |
Mouse embryonic stem cells (E14), macrophage cells (LM-1) and embryonic fibroblasts (NIH 3T3); human T lymphocytes (Jurkat) and embryonic kidney cells (HEK 239T) |
Screening of cell encapsulation ratios for different types of cells18
|
| Gelatin |
E. gracilis, C. reinhardtii
|
Chlorophyll and lipid accumulation79
|
| Agarose |
E. coli
|
MIC of rifampicin determination; mutant isolation84
|
| |
|
Molecular evolution
|
| W/O/W |
E. coli
|
Thiolactonases (100 fold increase)54
|
| Arysulfatase50
|
| β-Glucosidase (∼2 fold increase for lactose)23
|
| G-type nerve agent hydrolase (104-fold increase)24
|
| Cutinase (8 fold increase)87
|
| Esterase (2 fold increase)90
|
| Polymerase (1200 fold increase)92
|
| W/O/W |
B. subtilis
|
Protease (1.6 fold increase)22
|
| W/O/W |
S. cerevisiae
|
Endoglucanase-II cellulase (20 fold increase)88
|
| Glucose oxidase (5.8 fold increase)89
|
| Agarose-alginate polyelectrolyte |
E. coli
|
Phosphotriesterase (20 fold increase)93
|
| PEG |
E. coli
|
Hydrolytic enzyme (3 fold increase)94
|
| W/O/W |
Y. lipolytica
|
Riboflavin (54 fold increase)96
|
| W/O/W |
B. coagulans
|
Lactic acid (52% more effective)97
|
| |
|
Single-cell detection
|
| Agarose |
E. coli (O175:H7 and K12) |
Detection of O157:H7 (0.0001%)27
|
| Agarose |
Human gastric carcinoma cells (Kato III) and breast cancer cells (MDA-MB-231) |
Differentiation in gene expression level of a cancer biomarker (EpCAM)28
|
| Polyacrylamide-agarose |
E. coli XL1 |
Differentiation of a tetracycline resistance gene29
|
| Agarose |
T lymphocytes
|
Secretion of cytokines: IFN-γ, IL-4, IL-10 or TGF-β30
|
| Secretion of cytokines: IL-2, IFN-γ, TNF-α25
|
| |
|
Cell-to-cell interaction
|
| W/O/W |
S. aureu + microbes |
Antibiotic producer discovery32
|
|
S. aureus + S. venezuelae
|
|
S. aureus + E. coli
|
| W/O/W |
Oral microbiota of Siberian bear + S. aureus
|
Antibiotic Ami discovery31
|
| Agarose |
S. cerevisiae + S. aureus
|
Lytic hydrolase producer for S. aureus33
|
| Agarose |
C. sorokiniana + Pseudomonas spp. |
Viable bacteria improving algal growth34
|
| Agarose |
Stromal cells (MBA2) + leukemia cells (M07e) |
Impacts on M07e cells survival105
|
| Agarose |
S. cerevisiae + murine Ba/F3 reporter cells |
Cytokine secretion by S. cerevisiae libraries103
|
| Agarose |
P. pastoris + cancer cells (A431) |
Antibody secretion by P. pastoris libraries104
|
