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. 2022 Feb 18;121(7):1299–1311. doi: 10.1016/j.bpj.2022.02.025

Table 1.

Stability of individual Nedd4-2 mutant variants

Nedd4-2190−581 variant Tm (°C)
C209 54.2 ± 0.1
C218 55.3 ± 0.1
C389 53.6 ± 0.1
C414 55.7 ± 0.2
C508 51.06 ± 0.05
C522 56.5 ± 0.2
C571 54.4 ± 0.3
WT 56.29 ± 0.05
Nedd4-2186−975 variant Tm (°C)
WT 41.34 ± 0.06
C942 39.5 ± 0.4

Midpoint temperatures of the protein unfolding transition (Tm) for Nedd4-2 WT and mutants, as determined by differential scanning fluorimetry. Uncertainties are expressed as the SE values calculated from three experiments.