Table 1.
Nedd4-2190−581 variant | Tm (°C) |
---|---|
C209 | 54.2 ± 0.1 |
C218 | 55.3 ± 0.1 |
C389 | 53.6 ± 0.1 |
C414 | 55.7 ± 0.2 |
C508 | 51.06 ± 0.05 |
C522 | 56.5 ± 0.2 |
C571 | 54.4 ± 0.3 |
WT | 56.29 ± 0.05 |
Nedd4-2186−975 variant | Tm (°C) |
---|---|
WT | 41.34 ± 0.06 |
C942 | 39.5 ± 0.4 |
Midpoint temperatures of the protein unfolding transition (Tm) for Nedd4-2 WT and mutants, as determined by differential scanning fluorimetry. Uncertainties are expressed as the SE values calculated from three experiments.