Skip to main content
. 2022 Feb 23;121(7):1156–1165. doi: 10.1016/j.bpj.2022.02.036

Figure 2.

Figure 2

Measurements of pH in HeLa-SypHerRed cells under standard cultivation conditions. (A) Representative bright-field, fluorescence intensity and FLIM images of cells. Bar: 40 μm. (B) Scatter plot of fluorescence lifetimes versus intensity in the cytoplasm. Dots are the measurements in individual cells. (С) Enlarged area shown by the white square in (A). The dashed red, yellow, and blue lines indicate the areas selected for analysis. Note, that the small areas with short lifetime (∼0.7 ns, not pH-responsive) in the perinuclear zone were avoided upon ROI selection in the cytoplasm. (D) Quantification of pH in the cytoplasm (pHc), in the nucleus (pHn), and in the whole cell (pHi). Mean ± SD. N = 20–30 cells. To see this figure in color, go online.