Table 2.
Parameters of the mathematical model used to simulate concentration response data for the HCN4 channel expressed in HEK cells
| cAMP | Wild-type | S672R |
|---|---|---|
| q | 8.7 | 8.7 |
| Kd site 1 | 0.06 |
0.53 |
| Kd site 2 | 0.69 | 5.05 |
| Kd site 3 | 0.69 | 5.05 |
| Kd site 4 | 0.69 | 5.05 |
| k0c | 1 | 2.85 |
| K0 | 1 | 0.35 |
| 0.05 | 2 | |
| K1 | 1.11 | 1.11 |
| 0.07 | 0.8 | |
| K2 | 1.5 | 1.5 |
| 0.07 | 14 | |
| K3 | 1.7 | 1.7 |
| 0.03 | 100 | |
| K4 | 3.08 | 3.08 |
The table summarizes the sets of parameters of the mathematical model used for data and curves shown in Fig. 5B, showing the concentration of cAMP versus the depolarizing shift of the activation curve. The units of “q” are in mV, Kd values are in μM, while k and K are dimensionless. The value for q comes from the experimental data which were fit with the Boltzmann relation in (18).