Figure 2.

Identification of the hepatocyte intracellular proteome and hepatocyte secreted proteins. (A) Volcano plot showing hepatocyte intracellular proteins with those increased by exercise training denoted in blue and those decreased denoted in orange. (B) The dynamic range of quantified intracellular proteins. (C) Principal component analysis of the intracellular proteins. (D) Ingenuity Pathway Analysis showing enrichment of upstream factors in exercise-trained mice. (E) Canonical pathways within the hepatocyte. (F) Volcano plot showing hepatocyte secreted proteins with those increased by exercise training denoted in blue and those decreased denoted in orange. (G) The dynamic range of the quantified secreted proteins. (H) Principal component analysis of the hepatocyte secreted proteins. (I) Percentage of proteins that are classically or non-classically secreted. (J) Number of classically secreted proteins that are significantly increased or decreased with exercise training. (K–M) Ingenuity Pathway Analysis showing enrichment of exercise mediated changes in (K) diseases and biological functions, (L) canonical pathways and (M) upstream factors. (N) Correlation between exercise-training induced changes in intracellular proteome and corresponding secreted protein. Shown are means ± SEM, N = 7–8 per group. ∗P < 0.05 vs. Sedentary. Significance was assessed by two-way ANOVA followed by Bonferroni's post hoc test (panel B) or students two tailed unpaired t-test (panels C–I). Proteins identified by corresponding gene name.