Figure 3.

Impact of secreted factors derived from hepatocytes of sedentary and exercise-trained mice on metabolism. Hepatocyte-secreted factors from Sedentary or Exercise trained mice were applied to tissues followed by metabolic assessment. (A) Lipolysis and (B) insulin stimulated 2-deoxyglucose uptake in epididymal adipose tissue. (C) Insulin stimulated glucose uptake in soleus muscle. (D) Glucose output from precision cut liver slices. (E–I) Radiometric tracing of lipid metabolism in liver slices showing (E) fatty acid uptake, (F) triglyceride synthesis, (G) phospholipid synthesis, (H) total lipid synthesis, and (I) fatty acid oxidation. Data are presented as mean ± SEM, with n = 5–8 per group. ∗P < 0.05 vs. Sedentary. Significance was assessed by students two tailed unpaired t-test.