Figure 5.

Validation of Sdc4 overexpression in mice. (A) Schematic representation of the experimental design. C57BL/6 mice were fed a high fat diet (HFD) for 6 weeks then injected with Sdc4-AAV or Control-AAV. Following AAV administration, mice were maintained on a HFD for a further 6 weeks. (B)Sdc4 expression in liver, heart, kidney, skeletal muscle and epididymal adipose tissue. (C)Sdc1, Sdc2 and Sdc3 mRNA expression in the liver. (D) Targeted mass spectrometry analysis of SDC4 secretion from precision cut liver slices. Data are presented as mean ± SEM, with n = 4–10 per group. ∗P < 0.05 vs. Control-AAV. Significance was assessed by students two tailed unpaired t-test. Figure 5A was created with Biorender.com.