Figure 8.

SDC4 ectodomain regulates fatty acid metabolism and lipid droplet distribution.(A) Insulin stimulated glucose uptake with or without recombinant SDC4 in isolated soleus muscle. (B–D) Radiometric tracing of lipid metabolism in isolated hepatocytes with or without acute recombinant SDC4 treatment showing (B) fatty acid uptake, (C) total lipid storage and, (D) fatty acid oxidation. (E) Representative image of lipid droplet morphology stained with Bodipy, Mitotracker and DAPI. Scale bar denotes 10 μm. Hepatocytes were isolated from n = 3 mice, 5–8 hepatocytes were imaged across two technical replicates and quantified per mouse totalling n = 21 Control and n = 26 rSDC4 treated hepatocytes. (F) Quantification of the lipid droplet number within the cell, (G) the total area that lipid droplets occupy in each hepatocyte, and (H) the average lipid droplets area within the hepatocyte. (I) Proportion of different sized lipid droplets in isolated hepatocytes. Data are presented as mean ± SEM, ∗P < 0.05 vs. Control-AAV, with n = 4 per group for paired analyses. Significance was assessed by students two tailed unpaired t-test (panel B–E) or paired t-test (panel G–I).