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. 2022 Apr 13;20:101423. doi: 10.1016/j.tranon.2022.101423

Fig. 3.

Fig 3

TSPAN31 regulates the proliferation, tumor growth, and apoptosis of GC cells. (A and B) Determination of the expression level of TSPAN31 in GC cells transfected with siTSPAN31 and Lv-TSPAN31 OE by qRT-PCR. (C and D) The optical density (OD value) of GC cells transfected with si-TSPAN31 and Lv-TSPAN31 OE was measured at 450 nm by the CCK-8 assay. (E and F) The clonogenic of GC cells transfected with si-TSPAN31 and Lv-TSPAN31 OE was determined by the clone formation assay. (G and H) The apoptosis level of GC cells transfected with si-TSPAN31 and Lv-TSPAN31 OE was determined by the apoptosis assay. (I and J) The effect of si-TSPAN31 and LV-TSPAN31 OE transfection on GC tumor growth was detected by tumor bearing experiment in nude mice (*P < 0.05; ⁎⁎P < 0.01; ⁎⁎⁎P < 0.001).