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. 2022 Apr 13;20:101423. doi: 10.1016/j.tranon.2022.101423

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© 2022 Published by Elsevier Inc.

This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).

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Fig 6 — Low expression of TSPAN31 can partially reverse the inhibitory effect of high expression of METTL1 on the malignant phenotype of GC cells. (A) The mRNA expression of METTL1 after GC cells were co-transfected with METTL1 OE and shTSPAN31 was determined by qRT-PCR. (B) The OD value of GC cells co-transfected with METTL1 OE and shTSPAN31 was determined at 450 nm by the CCK-8 assay. (C) The clonogenic of GC cells co-transfected with METTL1 OE and shTSPAN31 was detected by the clone formation assay. (D) The lateral migration capacity of GC cells co-transfected with METTL1 OE and shTSPAN31 was determined by the wound healing assay (magnification: 100×). (E) The longitudinal migration capacity of GC cells co-transfected with METTL1 OE and shTSPAN31 was determined by the Transwell assay (magnification: 100×). (F) The apoptosis level of GC cells co-transfected with METTL1 OE and shTSPAN31 was determined by the apoptosis assay (*P < 0.05; ^⁎⁎P < 0.01; ^⁎⁎⁎P < 0.001).