. Author manuscript; available in PMC: 2023 Apr 1.

Published in final edited form as: Insect Biochem Mol Biol. 2022 Feb 16;143:103741. doi: 10.1016/j.ibmb.2022.103741

Table 1. List of 33 modifications detected in tRNA of mosquitoes.

tRNA modifications detected using LC-MS/MS. 33 modifications were detected in all female samples and are listed here. The abbreviation for the modification, the name, the molecular ion m/z ([MH⁺]), and retention time (RT) are listed here.

Modification	Name	MH⁺	RT (min)
Ψ	Pseudouridine	245.0773	1.6
D	Dihydrouridine	247.0930	1.5
m⁵C	5-methylcytidine	258.1090	4.8
m³C	3-methylcytidine	258.1090	3.4
Cm	2’-O-methylcytidine	258.1090	6.8
Ψm	2’-O-methylpseudouridine	259.0930	5.1
m³U	3-methyluridine	259.0930	13
Um	2’-O-methyluridine	259.0930	13
m⁵U	5-methyluridine	259.0930	8.5
I	Inosine	269.0886	8
Am	2’-O-methyladenosine	282.1202	29.5
m⁶A	N6-methyladenosine	282.1202	31.2
m¹A	1-methyladenosine	282.1202	4.2
m¹I	1-methylinosine	283.1042	18.3
ac⁴C	N4-acetylcytidine	286.1039	20.3
m⁶₂A	N6,N6-dimethyladenosine	296.1359	33.3
m²G	2-methylguanosine	298.1151	21.4
Gm	2’-O-methylguanosine	298.1151	17.9
m¹G	1-methylguanosine	298.1151	18.8
m⁷G	7-methylguanosine	298.1151	7.4
m²₂G	N2,N2-dimethylguanosine	312.1308	29.3
mcm⁵U	5-methylcarbonylmethyluridine	317.0985	18.8
mcm⁵s²U	5-methoxycarbonylmethyl-2-thiouridine	333.0756	30.3
i⁶A	N6-isopentenyladenosine	336.1672	36.5
acp³U	3-(3-amino-3-carboxypropyl)uridine	346.1250	2.8
acp³D	3-(3-amino-3-carboxypropyl)-5,6-dihydrouridine	348.1407	1.3
ms²i⁶A	2-methylthio-N6-isppentenyladenosine	382.1549	41.7
Q	Queuosine	410.1675	18.3
t⁶A	N6-threonylcarbamoyladenosine	413.1421	31.1
isomer oQ	Epoxyqueuosine	426.1625	16.4
m⁶t⁶A	N6-methyl-N6-threonylcarbamoyladenosine	427.1577	32.3
ms²t⁶A	2-methylthio-N6-threonylcarbamoyladenosine	459.1298	32.8
manQ	Mannosyl-queuosine	572.2204	19