Figure 6.

Senolytics increase α-Klotho in human urine; urinary SASP factors are inversely related to urinary α-Klotho. Subjects (n = 20) with idiopathic pulmonary fibrosis (IPF) were administered 3 courses of D+Q for 3 sequential days each (total 9 doses over 3 weeks). (a) Urinary α-Klotho and SASP factors were assayed at baseline and 5 days after the last D+Q treatment. Urinary α-Klotho in subjects with IPF was increased (Baseline: 293.49±115.48, Post-treatment: 392.79±95.24, normalized to urinary creatinine) by D+Q. Wilcoxon signed rank tests (two-sided) were used to test differences before and after treatment. *p = 0.04. (b–d) Spearman correlations of urinary α-Klotho and SASP factors are shown. FDR-corrected R² and p values are in Supplementary Table 2.