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. 2022 Apr 22;5(8):e202101303. doi: 10.26508/lsa.202101303

Figure 5. Knockdown of endogenous miR-26a and b counteracts pre-B cell expansion and enhances pre-B to immature B cell differentiation in vitro.

Figure 5.

(A) 1676 pre-B cells transduced either with a scrambled sponge (control) or with a miR-26 sponge construct were cultured without IL-7 for 72 h to induce differentiation. Contour plots depict the percentage of immature B cells as defined by muHC+kappa+ in transduced (dsRed+) and non-transduced (dsRed−) cells within one sample. The bar graph summarizes the ratio of immature B cells among transduced and non-transduced cells in primary bone marrow-derived B cell precursors and in the 1676 cell line. Lines connect the data points of the independent experiments. (B) 1676 pre-B cells expressing the miR-26 sponge or a scrambled sponge construct were cultured with IL-7 for 7 d. Representative histograms show the change in the percentage of transduced (dsRed+) cells over time. The bar graph provides a statistical analysis based on the cell growth index for the pre-B cell line 1676 and for primary B cell progenitors. Graphs show mean + SD and represent at least four independent experiments (A, B); groups were compared by a paired t test; **P < 0.01, ***P < 0.001.