Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2022 Apr 23;13:167. doi: 10.1186/s13287-022-02840-0

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© The Author(s) 2022

Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated in a credit line to the data.

PMC Copyright notice

Fig. 5 — a C57Bl6 mouse hearts were mounted on a Langendorff system. The ex vivo protocol comprises a 15 min-period of stabilization, followed by 30 min of global ischemia achieved by stopping the flow through the aorta (no-flow). Reperfusion was achieved by restoring the Tyrode perfusion during 60 min (IR group). In the MSC group, reperfusion was achieved with a solution of MSC cells prepared in a Tyrode buffer (2500 cells/mL) pre-treated or not by GW0742 at 1 µM. At the end of the protocol, hearts were collected and proteins extracted for further analysis. b, c, d Western blot analysis was performed from LV protein extracts from IR non-treated or MSC-treated murine IR hearts (ex vivo) with or without a pharmacological pre-treatment. Scatter dot blots and mean ± SD were plotted for Cleaved-caspase 3 (p** = 0.064 for IR, n = 10 vs MSC/GW0742, n = 10), for pAKT/AKT (p* = 0.0385 for IR, n = 8 vs MSC/GW0742, n = 8) and for pERK/ERK (p^ns = 0.2476 for IR, n = 6 vs MSC/GW0742, n = 6). Representative gel blots are presented for each protein for the three conditions (IR, MSC and MSC + GW0742). Vinculin or α-actinin were used as protein loading control. Data were compared using non parametric Kruskal–Wallis test (Dunn’s post hoc test)