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. Author manuscript; available in PMC: 2022 May 1.
Published in final edited form as: Neurobiol Dis. 2022 Jan 26;166:105637. doi: 10.1016/j.nbd.2022.105637

Fig. 2.

Fig. 2.

Quantification of chronic spontaneous convulsive seizures 2–4 wks post-IHKA

(A) Experimental timeline of the study. The 2–4 wk data were used in this figure (red arrows). For this timepoint, there were 9 mice (males, blue, gray, black or white; females, pink; total # seizures = 233).

(B) The total number of chronic convulsive seizures recorded 2–4 wks post-IHKA is shown per animal. Note that animals showed frequent convulsive seizures although there was variability. One of the female mice, animal #2, had 5 seizures in 2 wks which is fewer than other animals, but nevertheless is a demonstration of chronic epilepsy.

(C) The number of convulsive seizures is plotted for each day of the recording period. Each animal is a different line, has a different color designation, and was assigned a different number. The color coding is the same in subsequent figures to make it possible to compare each animal across figures.

(D) Convulsive seizure frequency was calculated as the mean number of convulsive seizures per day for each animal. Data are presented in D–F and H as individual values and as mean ± SEM (red).

(E) Convulsive seizure duration was calculated as the mean per animal (left) or the mean of all convulsive seizure durations (right; n = 211 seizures).

(F) Convulsive seizure severity was calculated as the mean per animal (left) or the mean of all convulsive seizures (right; n = 211 seizures).

(G) Convulsive seizure burden was defined as the percent of days spent with (SZ) or without (No SZ) seizures.

(H) The percent of convulsive seizures is shown, either occurring during the light period or dark period of the light:dark cycle (Lights ON or OFF; H1), a.m. or p.m. (AM, PM; H2), and in awake (AW) or sleep (SL) state (H3). The percentages were calculated as the mean per animal (H1a, H2a, H3a) or the mean of all seizures (H1b, H2b, H3b). There were no significant differences (paired t-tests; all p > 0.05) for the light:dark cycle or a.m. vs. p.m. However, a significantly higher percentage of seizures occurred during awake vs. sleep stage (paired t-test, tcrit = 2.416, p = 0.04).