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. 2022 Apr 20;42(16):3316–3328. doi: 10.1523/JNEUROSCI.2098-21.2022

Figure 5.

Figure 5.

Acute application of MOPr and DOPr agonists shows that pronociceptive actions are mediated by DOPr. A, B, Effect of low-concentration (10 nm) and high-concentration (10 μm) DAMGO applied acutely (30 min) on neuron excitability. Patch-clamp recordings show that low and high concentrations of DAMGO increased neuron rheobase. This antinociceptive effect was inhibited by MOPr antagonist CTOP (A: F(2,146) = 3.655, p = 0.028) but not by DOPr antagonist SDM25N (B: F(2,148) = 0.339, p = 0.713). C, D, A low concentration of DADLE increased neuron rheobase, whereas a high concentration decreased neuron rheobase. DOPr antagonist SDM25N blocked both effects induced by DADLE (C: F(1,134) = 8.35, p = 0.0004), whereas CTOP was unable to block the pronociceptive effect evoked by high-concentration DADLE (D: F(1,54) = 0.1555, p = 0.695). Number of cells appears in each bar. Error bars indicate mean ± SEM. *p ≤ 0.05; **p ≤ 0.01; ***p ≤ 0.001; two-way ANOVA with Bonferroni's post hoc test.