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. 2022 Apr 20;42(16):3316–3328. doi: 10.1523/JNEUROSCI.2098-21.2022

Figure 6.

Figure 6.

DOPr mediates the pronociceptive effect induced by high concentrations of MOPr and DOPr agonists applied overnight. A, B, Effect of low-concentration (10 nm) and high-concentration (10 μm) DAMGO overnight incubated on DRG neuron excitability. DRG neuron rheobase increased with low-concentration but decreased with high-concentration DAMGO. A, CTOP prevented the antinociceptive effect by 10 nm DAMGO but had no effect on the pronociceptive effect by 10 μm DAMGO (F(2,127) = 9.898, p < 0.0001). B, SDM25N inhibited only the pronociceptive effect by 10 μm DAMGO (F(2,71) = 8.144, p = 0.0007). C, D, Low-concentration DADLE had no effect on neuron rheobase, while high concentrations decreased neuron rheobase; this pronociceptive effect by high-concentration DADLE was inhibited by both SDM25N (C: F(2,161) = 5.022, p = 0.0077) and CTOP (D: F(1,72) = 5.605, p = 0.0206). E, Effect of 10 μm DADLE on DRG neurons innervating the colon of female mice. High-concentration DADLE decreased the rheobase of Fast Blue-labeled neurons (t(14) = 3.506, p = 0.0035, unpaired t test). Number of cells appears in each bar. Error bars indicate mean ± SEM. A–D, *p ≤ 0.05; **p ≤ 0.01; ***p ≤ 0.001; two-way ANOVA with Bonferroni's post hoc test.