Fig. 3.

Time course of proportion of diffusible protein in muscle fibres from control and T2D individuals. Diffusion of glycogen (A), AMPK-β2 (B), and glycogen-related proteins (glycogen debranching enzyme (GDE) (C), glycogen branching enzyme (GBE) (D) and glycogen phosphorylase (GP) (E) in 1-, 10- and 30-min wash times to isolate diffusible proteins located within the muscle fibre. Data are expressed as means ± SD where the amount of protein diffused out into the wash solution is expressed as a percentage of the total (% of the wash and fibre (total)). n signifies the total number of fibre wash sets. Statistical comparison shown in table, one-way ANOVA within population, Bonferroni’s post hoc analyses to assess 1 vs 10 min and 10 vs 30 min timepoints (except GP, unpaired t test). Closed squares are T2D and open squares are CON