KEY RESOURCES TABLE
| REAGENT or RESOURCE | SOURCE | IDENTIFIER |
|---|---|---|
| Antibodies | ||
| Chicken polyclonal anti-NeuN | Millipore Sigma | Cat#:ABN191, RRID:AB_11205760 |
| Chicken polyclonal anti-GFP | ThermoFisher Scientific | Cat#:A10262, RRID:AB_2534023 |
| Guinea pig polyclonal anti-NeuN | Millipore Sigma | Cat#:ABN90, RRID:AB_11205592 |
| Mouse monoclonal anti-HA | BioLegend | Cat#:901513, Clone ID: 16B12, RRID:AB_2565335 |
| Mouse monoclonal anti-NeuN | Millipore Sigma | Cat#:MAB377, RRID:AB_2298772 |
| Rabbit polyclonal anti-Arc | Synaptic Systems | Cat#:156003, RRID:AB_887694 |
| Bacterial and Virus Strains | ||
| rAAV-CaMKIIα-eArchT3.0-eYFP | UNC Vector Core | Cat#:AV4883 |
| rAAV-CaMKIIα-eYFP | UNC Vector Core | Cat#:AV4808c |
| pAAV-CaMKIIα-hM4D(Gi)-mCherry | Bryan Roth (unpublished) | Cat#:50477, RRID:Addgene_50477 |
| pAAV-CaMKIIα-mCherry | UNC Vector Core | Cat#:AV4809D |
| pAAV-PRAM-d2tTA:TRE-NLS-mKate2-WPREpA | Sorensen et al., Elife, 2016 | Cat#:84474, RRID:Addgene_84474 |
| pAAV-PRAM-d2tTA:TRE-HA-hM4D(Gi)-WPREpA | This paper | N/A |
| pENN.AAV.CamKII.GCaMP6f.WPRE.SV40 | Penn Vector Core | Cat#100834, RRID:Addgene_100834 |
| pAAV-hSyn-hM4D(Gi)-mCherry | Bryan Roth (unpublished) | Cat#:50475, RRID:Addgene_50475 |
| pAAV-hSyn-mCherry | Karl Deisseroth (unpublished) | Cat#:114472, RRID:Addgene_114472 |
| Chemicals, Peptides, and Recombinant Proteins | ||
| Cholrea Toxin Subunit B, Alex Fluor 555 | ThermoFisher Scientific | C22843 |
| Clozapine N-Oxide | Enzo Life Sciences | BML-NS105-0025, CAS: 34233-69-7 |
| Clozapine N-Oxide-dihydrochloride | HelloBio | HB6149, CAS: 2250025-93-3 |
| DAPI (4’,6-Diamidino-2-Phenylindole, Dihydrochloride) | ThermoFisher Scientific | D1306, CAS: 28718-90-3 |
| Dil | Biotium | Cat#60010, CAS: 41085-99-8 |
| NMDA (N-Methyl-D-aspartic acid) | Sigma | M3262, CAS:6384-92-5 |
| (RS)-CPP | Tocris | Cat#0173, CAS: 100828-16-8 |
| VECTASHIELD® Antifade Mounting Medium | Vector Laboratories | H-1000, RRID:AB_2336789 |
| Critical Commercial Assays | ||
| In-Fusion HD Cloning Plus kit | Takara | 638920 |
| Experimental Models: Organisms/Strains | ||
| C57BL/6J | Jackson Labs | Cat#:000664, RRID:IMSR_JAX:000664 |
| Software and Algorithms | ||
| Behavioral Observation Research Interactive Software (BORIS) | Friard & Gamba, Methods in Ecology and Evolution, 2016 | http://www.boris.unito.it/, RRID:SCR_021434 |
| Clampex 11.2 | Molecular Devices | https://www.moleculardevices.com/products/axon-patch-clamp-system/acquisition-and-analysis-software/pclamp-software-suite, RRID:SCR_011323 |
| Clampfit 11.2 | Molecular Devices | https://www.moleculardevices.com/products/axon-patch-clamp-system/acquisition-and-analysis-software/pclamp-software-suite, RRID:SCR_011323 |
| Custom MATLAB Code | This paper | https://zenodo.org/record/5838961, DOI: 10.5281/zenodo.5838961 |
| ImageJ | NIH | https://imagej.nih.gov/ij/, RRID:SCR_003070 |
| Inscopix Data Processing Software 1.2.1 | Inscopix | N/A |
| MATLAB | MathWorks | https://www.mathworks.com/products/matlab.html?s_tid=hp_products_matlab, RRID:SCR_001622 |
| Mosaic 1.2.0 | Inscopix | RRID:SCR_017408 |
| Prism 9 | GraphPad | https://www.graphpad.com/scientific-software/prism/, RRID:SCR_002798 |
| Video Freeze | Med Associates | https://www.med-associates.com/product/video-fear-conditioning/, SOF-843, RRID:SCR_014574 |
| Zen Blue 2.3 | Zeiss | https://www.zeiss.com/microscopy/int/products/microscope-software/zen.html,RRID:SCR_021725 |
| Zen Lite | Zeiss | https://www.zeiss.com/microscopy/int/products/microscope-software/zen.html, RRID:SCR_021725 |
| Other | ||
| 10 μL Microliter Syringe Model 701 | Hamilton | 80301 |
| 3-D Printed Custom-Designed Microdrive | This paper | N/A |
| 532 nm Laser | Ultralasers | CST-w532–500mW |
| Axon Digidata 1550B | Molecular Devices | N/A |
| Baseplate | Inscopix | 1050–004638 |
| C&B Metabond Quick Adhesive Cement System | Parkell | S380 |
| Capillary Borosilicate Glass 1.5mm OD, 0.84mm ID, 6 inch length, with filament | World Precision Instruments | 1B150F-4 |
| Dense Fabric Ball Attached to String | This paper | N/A |
| Digital Lynx 4SX | NeuraLynx | N/A |
| Doxyclyine mouse food 40 mg/kg, custom | Bio-serv | F5003 |
| Elevated Platform for Unrelated Aversive Experience (Bright Light Illumination) | This paper | N/A |
| Function Generator | Siglent | SDG810 |
| Inline Heater | Warner Instruments | SH-27B |
| Lecia VT1000 S Vibrating Blade Microtome | Leica | VT1000 S |
| Leica S6E Stereomicroscope | Leica | S6E |
| Microelectrode Holder (MPH6S) | World Precision Instruments | MPH6S10 |
| MicroFil | World Precision Instruments | MF28G-5 |
| Microinjection Syringe Pump | World Precision Instruments | UMP3T-1 |
| Mono Fiber Optic Cannula | Doric Lenses | MFC_200/240-0.22_50mm_ZF1.25(G)_FLT |
| MultiClamp 700B Microelectrode Amplifier | Molecular Devices | N/A |
| NIR Video Fear Conditioning Package for Mouse | Med Associates | MED-VFC-OPTO-USB-M |
| NOA81 – Fast Curing Optical Adhesive | Norland | NOA81 |
| nVoke v1.0 | Inscopix | N/A |
| Observational Fear Apparatus | This paper | N/A |
| Pipette Puller | Narishige | PC-100 |
| ProView Lens Probe | Inscopix | 1050-004608 |
| ProView Prism Probe | Inscopix | 1050-004612 |
| Single Channel Temperature Controller | Warner Instruments | TC-324C |
| Small Animal Stereotaxic Instrument with Digital Display Console | David Kopf Instruments | Model 942 |
| UV Curing LED System, 365 nm | ThorLabs | CS2010 |
| Wiretrol® I | Drummond Scientific Company | 5-000-1001-X |
| Zeiss Axio Examiner A1 | Zeiss | N/A |
| Zeiss AxioImager M2 with Apotome | Zeiss | N/A |
| Zeiss LSM800 with Airyscan | Zeiss | N/A |