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. Author manuscript; available in PMC: 2022 May 1.
Published in final edited form as: Hepatology. 2022 Feb 8;75(5):1110–1122. doi: 10.1002/hep.32298

FIG. 1.

FIG. 1.

Ciliogenesis is impaired in PLDC grown under conventional conditions. (A) A schematic outline of a conventional model of ciliogenesis in vitro. (B) Representative immunofluorescence confocal microscopy (IF) images and (C) a quantitative analysis show that PLDC cilia (detected by a ciliary marker, acetylated α-tubulin, stained in red) at day 7 post-confluence are shorter compared to NHC cilia. Nuclei (blue, DAPI). Data are presented as Mean±SD. n=10 cilia for each experimental condition. **p<0.001; ****p<0.0001.