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. Author manuscript; available in PMC: 2022 May 1.

Published in final edited form as: Hepatology. 2022 Feb 8;75(5):1110–1122. doi: 10.1002/hep.32298

FIG. 7. — Inhibition of autophagy by Mefloquine (MQ) and verteporfin (VP) reestablishes PLDC cilia and ciliary localization of ARL13B, ARL3, and TGR5. (A, C) MQ and VP increased the length of cilia (red) in cultured PLDC by 3-fold, and (B, D) in PCK rats by 2-fold. In PCK rats treated with DMSO (control), Arl13b (magenta) and TGR5 (red) (E) and Arl3 (magenta) and TGR5 (red) (F) are depleted in cholangiocyte cilia (green, acetylated α-tubulin). In response to treatment with MQ and VP, ciliary localization of Arl13b, Arl3, and TGR5 was reestablished. Scale bar, 5 μm. Nuclei (blue, DAPI). Data are presented as Mean±SD. n=10 cilia for each experimental condition; ****p<0.0001.

FIG. 7. — Inhibition of autophagy by Mefloquine (MQ) and verteporfin (VP) reestablishes PLDC cilia and ciliary localization of ARL13B, ARL3, and TGR5. (A, C) MQ and VP increased the length of cilia (red) in cultured PLDC by 3-fold, and (B, D) in PCK rats by 2-fold. In PCK rats treated with DMSO (control), Arl13b (magenta) and TGR5 (red) (E) and Arl3 (magenta) and TGR5 (red) (F) are depleted in cholangiocyte cilia (green, acetylated α-tubulin). In response to treatment with MQ and VP, ciliary localization of Arl13b, Arl3, and TGR5 was reestablished. Scale bar, 5 μm. Nuclei (blue, DAPI). Data are presented as Mean±SD. n=10 cilia for each experimental condition; ****p<0.0001.