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. Author manuscript; available in PMC: 2022 May 1.
Published in final edited form as: Mitochondrion. 2022 Mar 17;64:59–72. doi: 10.1016/j.mito.2022.03.001

Fig. 5.

Fig. 5.

Analysis of interacting mutations in nuoM of E. coli. (A) The locations of mutations nuoN_F396A (orange) and nuoM_L190T (blue) are shown in nuoN (light blue) and nuoM (light yellow), using PDB file 3rko (Efremov and Sazanov, 2011). (B) dNADH-oxidase activity of membrane vesicles prepared from the E. coli double mutant nuoN_F396A/nuoM_L190T is shown compared to a wild type sample prepared the same day. (C) Proton translocation rates from the same samples shown in panel B are indicated by fluorescence quenching of the acridine dye ACMA. (For interpretation of the references to color in this figure legend, the reader is referred to the web version of this article.)