Table 4.
De-cellularized ECM and their applications for in situ regeneration
Biomaterial | De-cellularization reagents | Animal model | Outcome | Refs. |
---|---|---|---|---|
Porcine esophageal mucosa | 1% trypsin/0.05% EDTA/37 °C/1h; 3% Triton X-100/48h | Rat abdmoninal esophagus defect | Oesophagal mucosa reconstruction | [63] |
Porcine dermal tissue | 2% sodium deoxycholate/10mM HEPES/24h; DNAse overnight/4C | Primate abdominal wall excision repair | Repopulation & maturation of vascular cells; transient immune response | [64] |
Porcine urinary bladder matrix | 0.1% peracetic acid; 4% ethanol/2h | Dog hemilarynx defect | Re-epithelialization; formation of cartilageous structure; normal phonation; scarred regenerated vocal mucosa | [65] |