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. Author manuscript; available in PMC: 2023 May 1.
Published in final edited form as: J Thromb Haemost. 2022 Feb 27;20(5):1182–1192. doi: 10.1111/jth.15670

Figure 3: TG2-cross-linked fibrin(ogen) selectively attenuates early LPS-induced IL-10 expression.

Figure 3:

BMDMs were cultured on an uncoated surface, fibrinogen-coated surface, or TG2-cross-linked fibrinogen-coated surface for 4 hours then stimulated with lipopolysaccharide (LPS) for the indicated time points. (A) Tnfa and (B) Il10 expression was measured in BMDMs treated with 1 ng/mL LPS for 30, 60, or 120 min via qRT-PCR. Data are presented as mean fold induction + SEM compared to vehicle-treated BMDMs on an uncoated surface at t=0 (not shown) (n=3–11). (C) TNFα, (D) IL-6, and (E) IL-10 protein levels were measured in supernatant following stimulation of BMDMs with 1 ng/mL LPS for two hours (n=7–13). *p<0.05, **p<0.01, ***p<0.001, ****p<0.0001