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. Author manuscript; available in PMC: 2022 Oct 7.
Published in final edited form as: Nat Cell Biol. 2022 Apr 7;24(4):513–525. doi: 10.1038/s41556-022-00872-5

Extended Data Fig. 2. CycT1 but not CDK9 in P-TEFb is PARylated in response to treatment with MNNG.

Extended Data Fig. 2

a, Schematic diagram depicting different regions on the PAR polymer that are recognized by pan-ADPr, αpADPr (10H) and pADPr, respectively. b, CycT1 was affinity-purified from HeLa cells either untreated or treated with MNNG and analyzed by Western blotting (WB) with pan-ADPr and αpADPr (10H). c, CDK9 was purified from HeLa cells, which were untreated or treated with MNNG, and analyzed by WB. d, HeLa or F1C2 cells stably expressing CDK9-F were treated with MNNG. Anti-Flag immunoprecipitates (IP) were analyzed by WB for the indicated proteins. All Western blots are representative of three independent experiments. Gel source data are available online.