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. Author manuscript; available in PMC: 2023 Apr 1.
Published in final edited form as: Adv Healthc Mater. 2022 Feb 18;11(8):e2102265. doi: 10.1002/adhm.202102265

Figure 3. Collagenase digestion and retention of dECM samples.

Figure 3.

(A) dECM microparticles and hydrogels were digested by collagenase for 24 h. (B) dECM hydrogels were completed digested within 2 h, while the complete digestion of dECM microparticles took more than 24 h. (C) dECM microparticles and dECM hydrogel precursor labeled by WGA-Alexa Flour 488 were injected into porcine heart tissue explants cultured for 14 days. (D) Marginal levels of dECM hydrogel precursor can be observed on day 14. The areas of labeled dECM microparticles decreased by 50% from day 1 to day 14. (E) The average fluorescence intensity of the remaining samples decreased by 30% in dECM microparticles and 60% in dECM hydrogel precursor. (Panel B: 3 repeats per group. Panel D, E: 3 repeats per group, unpaired t-test. *p<0.05, **p<0.01. All data presented as mean ± SD. Panel A: dash line circles dECM.)