Figure 7. dECM microparticles stimulate cardiomyocyte cell cycle activity in post-MI hearts.
(A) Cardiomyocyte cell cycle activity was examined by immunostaining for Ki67 expression and BrdU incorporation in TnT-positive cells. (B) dECM microparticles significantly increased the percentage of Ki67+ cardiomyocytes compared to MI-control. (C) Both dECM microparticles and dECM hydrogel precursor promoted significant cardiomyocyte BrdU incorporation compared to MI-control. (Panel B, C: n=4 per treatment, one-way ANOVA and Tukey’s test. *p<0.05, **p<0.01. All data presented as mean ± SD. Arrows indicate mitotic cardiomyocytes; white boxes indicate magnified areas. Single channels of representative images in panel A are shown in Figure S6D, E.)