Figure 5. Effect of bafilomycin, DFP, or both on exosome fraction mtDNA-templated mRNA transcripts.
(A) Representative ethidium bromide-stained agarose gel showing amplified mtDNA-templated mRNA transcripts (humanin, ND2, and CO2) as well as amplified β-actin. (B) Relative levels of humanin cDNA, normalized to β-actin cDNA, for each condition. (C) Relative levels of ND2 cDNA, normalized to β-actin cDNA, for each condition. (D) Relative levels of ND2 cDNA, normalized to β-actin cDNA, for each condition. Data represent means ± SEM, *p < 0.05, **p < 0.01, ***p < 0.001, as analyzed through one-way ANOVA with Tukey’s multiple comparisons test; significant changes relative to the no-treatment control (Ctrl), and between the DFP and bafilomycin+DFP conditions, are indicated.