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. 2022 Apr 23;13(4):399. doi: 10.1038/s41419-022-04856-z

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© The Author(s) 2022

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 5 — The plasmid and siRNA of Atg7 were transfected into BUMPT cells and treated with or without 5 ng/ml TGF-β1 for 24 h. A, C Immunoblot analysis of Atg7 & LC3 & p62 & FN & Collagen I & Collagen III and Vimentin in BUMPT cells. B, D Analysis of the grayscale image between them. #P < 0.05 versus NC or Vector with the control group. *P < 0.05 versus NC or Vector with the TGF-β group. E After GFP-LC3 transfection, HK-2 cells were transfected with or without the plasmid or siRNA of ATG7, followed by the treatment with TGF-β1 for 24 h. The punctate accumulation of GFP-LC3 was visualized under fluorescence microscopy. F The number of LC3 puncta per cell was calculated. #P < 0.05 versus NC or Vector with the control group. ^P < 0.05 versus NC or Vector with the TGF-β group. *P < 0.05 versus NC or Vector with the TGF-β group. Each experiment A, C, E was repeated six times independently with similar results. B, D, F indicate the statistical Student’s t test used (means ± s.d., n = 6, P < 0.05).