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. 2022 Apr 23;12:6674. doi: 10.1038/s41598-022-10692-0

Figure 4.

Figure 4

Asteltoxin induces nuclear localization of MiT/TFE transcription factor family members and promotes lysosome function. (A) PC3 cells were treated with DMSO, 10 µg/mL asteltoxin, or 200 nM rapamycin for 24 h and were immunostained using anti-TFE3. Scale bar = 20 μm. (B) Quantification of the ratio (%) of cells with nuclear localization of TFE3 (n > 77 cells, pooled from three independent experiments). (C) PC3 cells were treated with DMSO, 10 μg/mL asteltoxin, or 200 nM rapamycin for 24 h, and expression of lysosomal genes was analyzed. Statistical analysis was performed using one-way ANOVA. *P < 0.05 and **P < 0.01.