Skip to main content
. Author manuscript; available in PMC: 2022 Apr 24.
Published in final edited form as: Bone. 2020 Sep 25;143:115657. doi: 10.1016/j.bone.2020.115657

Figure 1: JAG1 delivery in a PEG hydrogel stimulates bone regeneration in a critical-sized bone defect mouse model.

Figure 1:

As a proof of concept experiment, we (A) incorporated JAG1-dynabeads complex (5 μM, 10 μM or 20 μM), dynabeads alone and BMP2 (2.5 μM) in 4% PEG-MAL hydrogels and implanted them into (B-G) 3.5 mm critical-sized defects in the parietal bones of 6–8-week old C57BL/6 mice and delivered them (J) without or (K) with CNC cells into the defects as 3 separate doses (Initial dose, Week 4, Week 8), as shown in H. After 12 weeks, we quantified differences in regenerated bone volume (J-K) within the defect and compared them between experimental groups by μCT analysis. μCT reconstructions of defects are shown in I. Data were subjected to ANOVA and Tukey’s post-test and are presented as mean (n ≥ 2) ± SD with p values reported.