Figure 5. Kr-h1 represses caste-inappropriate genes.
(A) RT-qPCR for Kr-h1 in the brains of immature workers (left) or gamergates (right) injected with siRNAs against Kr-h1 or GFP. Data are normalized to the housekeeping gene Rpl32 and shown as the ratio to the GFPi control. Bars represent the mean + SEM. P-values are from Student’s t tests.
(B) The MA plots show differential expression upon Kr-h1 knockdown in immature workers or gamergates. Colors highlight genes with caste-biased Kr-h1 binding in workers (left, green) or gamergates (right, blue). P-values are from Fisher’s exact tests for the gene set overlaps.
(C) Differential expression upon Kr-h1 knockdown in immature workers (left) or gamergates (right). Genes were classified according to their Kr-h1 binding preference in the brains of the mature castes as in Fig. 4D–E. P-values are from Mann-Whitney U tests.
(D) Differential expression in mature workers and gamergates. Genes were classified according to the effect of Kr-h1 knockdown in immature workers (left) or gamergates (right). P-values are from Mann Whitney U tests.
(E and F) Expression levels (DESeq2-normalized counts) after Kr-h1 knockdown and in neuronal cultures. Bars represent mean + SEM. P-values are from Student’s t tests.