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. Author manuscript; available in PMC: 2022 Nov 15.
Published in final edited form as: Cell. 2021 Nov 4;184(23):5807–5823.e14. doi: 10.1016/j.cell.2021.10.006

Figure 7. JH3 and 20E regulate the genomic distribution of Kr-h1.

Figure 7.

(A–C) Overlap of genes with peaks for Met (top), EcR (bottom), and Kr-h1 (A), genes with caste-biased expression and bound (B) or not (C) by Kr-h1. Data are from 6 replicates (3 workers and 3 gamergates) for both Met and EcR. For peak determination worker and gamergate data were combined. Kr-h1 peaks are from Fig. 4. P-values are from Fisher’s exact tests.

(D) Overlap of genes bound by Met (light green) or genes bound by EcR without Met (light blue) with worker-biased (left) or gamergate-biased (right) genes. Top: odds ratios for the overlaps. Bottom: number of overlapping genes in each category.

(E) Metaplots for Kr-h1 occupancy (RPMs) on the top 500 differentially bound regions across castes ± 1 kb, after head injections of JH3 (green) or 20E (blue). Data are from 2 biological replicates (injections) per condition.

(F) The ChIP-seq MA plot shows differential binding of Kr-h1 after injection of JH3 or 20E, as in (E). Colors highlight peaks with more Kr-h1 bound in workers (green) or gamergates (blue). P-values are from Fisher’s exact tests for the overlaps.

(G) Proposed model.

See also Figure S7.