Fig. 4.

Magnetofluidic instrumentation and PCR. A – Schematic of internal instrumentation with servo-actuated magnet arm, thermoelectric (TE) PCR heat block, and CMOS camera-based fluorescence imaging with LED illumination. B – Temperature readout from thermistor attached to PCR heat block during thermocycling. 40 cycles complete in under 13 minutes. C – Isolated images of PCR well in both FAM (HIV) and Cy5 (RP) channels with corresponding baseline-subtracted real-time fluorescence curve reporting average pixel intensities at each cycle.