REAGENT or RESOURCE	SOURCE	IDENTIFIER
Chemicals, peptides, and recombinant proteins
Coelenterazine	GoldBio	Cat#: CZ2.5, CAS: 55779-48-1
Dulbecco’s Modified Eagles Medium (DMEM)	Gibco	Cat#: 11965-092
Fetal Bovine Serum (FBS)	Sigma	Cat#: F1051
Penicillin-streptomycin	HyClone	Cat#: SV30010
Dulbecco’s Phosphate Buffer Saline	Sigma	Cat#: D5652-10X1L
0.05% Trypsin + 0.53 mM EDTA	Corning	Cat#: 25-052-CI
Deposited data
NT50 Values and De-identified patient data	SeroNet Coordinating Center, NCI, NIH	N/A
Experimental models: Cell lines
HEK293T	ATCC	Cat#: CRL-11268, RRID: CVCL_1926
HEK293T-ACE2	BEI Resources	Cat#: NR-52511, RRID: CVCL_A7UK
Oligonucleotides
CDC N1 F: 5′-GACCCCAAAATCAGCGAAAT-3′	Sigma-Aldrich	This paper
CDC N1 R: 5′-TCTGGTTACTGCCAGTTGAATCTG-3′	Sigma-Aldrich	This paper
CDC N2 F: 5′-TTACAAACATTGGCCGCAAA-3′	Sigma-Aldrich	This paper
CDC N2 R: 5′-GCGCGACATTCCGAAGAA-3′	Sigma-Aldrich	This paper
Recombinant DNA
pNL4-3-inGluc	David Derse, NCI, NIH Mazurov et al. (2010)	N/A
pcDNA3.1-SARS-CoV-2-Flag-S-Flag_D614G	GenScript Biotech, Zeng et al. (2021a)	N/A
pcDNA3.1-SARS-CoV-2-Flag-S-Flag_B.1.1.7	GenScript Biotech Zeng et al. (2021a)	N/A
pcDNA3.1-SARS-CoV-2-Flag-S-Flag_B.1.351	GenScript Biotech, Zeng et al. (2021a)	N/A
pcDNA3.1-SARS-CoV-2-Flag-S-Flag_B.1.617.2	GenScript Biotech, Evans et al. (2022)	N/A
pcDNA3.1-SARS-CoV-2-Flag-S-Flag_BA.1.1	GenScript Biotech, This paper	N/A
pcDNA3.1-SARS-CoV-2-Flag-S-Flag_BA.1.1-ΔEPE	GenScript Biotech, This paper	N/A
pcDNA3.1-SARS-CoV-2-Flag-S-Flag_BA.1	GenScript Biotech, This paper	N/A
pcDNA3.1-SARS-CoV-2-Flag-S-Flag_BA.2	GenScript Biotech, This paper	N/A
Software and algorithms
GraphPad Prism	Version 9.0.0	GraphPad