Fig. 5.

a. Western blot analysis of the lung tissue protein expression of phosphorylated phosphoinositide 3–kinase (PI3K), protein kinase B (Akt) and Fork head box protein O1 (FoxO1) in the whole cell lysates in CP-induced lung toxicity in rats. β-actin was used as an internal loading control Quantified expression of b. p-Akt; c. p-PI3K and d. p-FoxO. Data are represented as the means ± standard error of mean (SEM); analyzed by One-way ANOVA followed by Tukey’s multiple comparisons test. A level of probability (P value) ≤ 0.05 was considered significant. * Significant versus Control group; # significant versus CP-treated group. CP: cyclophosphamide; ALO: alogliptin