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. 2022 Apr 25;79(5):257. doi: 10.1007/s00018-022-04190-9

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© The Author(s) 2022

Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 1 — Genomic regions of SCNN1B investigated for methylation analysis in CFBE and CF primary bronchial epithelial cells. The first zone investigated, the pre-island, covers the region from -673 to -392, contains 56 cytosines and is located upstream to the CpG island. The second zone studied, spans the CpG island, covers the region from − 370 to + 372, contains 236 cytosines and includes the exon 1 and the TSS