. 2022 Apr 25;79(5):257. doi: 10.1007/s00018-022-04190-9

Table 2.

Primers used for DNA methylation analysis

Region	Forward	Reverse	T_a
Pre-island	5ʹ-GTGG^C/_TTGAAATGATAGT^C/_T^C/_TTGAAA^C/_T^C/_TTT-3ʹ	5ʹ-CACCCCT^G/_ACA^G/_AACACA^G/_AT^G/_ATCCTCT^G/_A-3ʹ	58 °C
Island	5ʹ-^C/_TTTG^C/_T^C/_T^C/_TAGAGGA^C/_TA^C/_TTGTGT^C/_TTG^C/_TAGG-3ʹ	5ʹ-TCT^G/_ACACCCT^G/_A^G/_A^G/_A^G/_A^G/_ACTTTTCCCA^G/_AC-3ʹ	58 °C