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. Author manuscript; available in PMC: 2022 Nov 14.
Published in final edited form as: ACS Nano. 2021 Oct 25;15(11):18374–18384. doi: 10.1021/acsnano.1c07856

Figure 1. Design of nanoparticle-SpyCatcher-scFv antibody conjugates.

Figure 1.

A) Structure of full IgG Ab (PDB file 1HZH).6 Variable chains that bind antigens and are used to engineer miniaturized scFv Ab are highlighted. B) Schematic of NP-SpyCatcher-scFv Ab conjugates. Core/shell nanocrystals (red and dark gray), encapsulated in amphiphilic polymers (light gray), conjugated to a single-Cys SpyCatcher (PDB file 4MLI)23 and SpyTag-scFv (PDB file 5YD5)24 chimera. The SpyTag peptide is added at the scFv C terminus, marked (*) in the structure.