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. Author manuscript; available in PMC: 2022 Nov 14.
Published in final edited form as: ACS Nano. 2021 Oct 25;15(11):18374–18384. doi: 10.1021/acsnano.1c07856

Figure 2. Synthesis of scFv Ab-conjugated aUCNPs.

Figure 2.

(A) Expression and reaction of SpyCatcher (SC) and SpyTag-scFv Ab (ST-scFv), monitored by SDS-PAGE. Partially purified His-tagged scFv were incubated with an excess of SC. (B) MALDI mass spectra of purified SC and ST-scFv before and after conjugation. (C) High-angle annular dark field transmission electron micrograph of core/shell NaEr0.8Yb0.2F4 @ NaY0.8Gd0.2F4 aUCNPs. Scale bar is 50 nm. (D) Diameters of nanoparticles determined by dynamic light scattering (DLS), of hydrophobic (blue), PMAO-encapsulated (teal), SpyCatcher-functionalized (yellow), and scFv-conjugated (red) UCNPs. Mean diameters (n = 5) are 19, 21, 24, and 28 nm, respectively.