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. Author manuscript; available in PMC: 2022 May 26.
Published in final edited form as: Chem Rev. 2021 Oct 25;122(10):9848–9879. doi: 10.1021/acs.chemrev.1c00662

Figure 12.

Figure 12.

1H-1H distance experiments restrain the structure of membrane proteins. (A) Residue-specific 1H-labeling (proton clouds) greatly reduces spectral congestion, as demonstrated on ubiquitin142. (B) Application of the proton cloud approach to structural studies of BamA142 (PDB 4K3B). (C) Solid-state NMR structure of the β-barrel OmpG (PDB 5MWV) in E. coli lipid extracts143. (D) Solid-state NMR structure of the β-barrel AlkL (PDB 6QWR) in DMPC bilayers, determined using distance restraints obtained from 1H-1H RFDR and BASS-SD144.