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TSAR experiments for measuring long-range 13C-13C and 13C-15N distances to determine amyloid protein structures. (A) 12.5 ms 13C-13C PULSAR spectra of glucagon helped to determine antiparallel packing of this amyloid fibril194,206 (PDB 6NZN). (B) 20 ms 2D 13C PAR spectrum helped to determine the Aβ42 fibril structure211 (PDB 5KK3). (C) 6 ms 2D 15N-13C PAINCP spectrum of RIPK1-RIPK3 necrosome fibrils and the resulting structure217 (PDB 5V7Z).
