TABLE 1.
Quantitative measurement of histone-associated DNA fragmentation in monolayers of porcine proximal tubular cells (LLC-PK1) by photometric ELISA
| Drug application groupa | % DNA fragmentationb ± SD |
|---|---|
| No AmB | 1.0 ± 0.34 |
| No AmB plus 100 μmol of caspase-3 inhibitor | 0.8 ± 0.23 |
| AmB (0.5 μg/ml) | 22.0 ± 0.35* |
| AmB (0.5 μg/ml) plus 100 μmol of caspase-3 inhibitor | 1.5 ± 0.26 |
| AmB (1.0 μg/ml) | 41.0 ± 1.36* |
| AmB (1.0 μg/ml) plus 100 μmol of caspase-3 inhibitor | 1.0 ± 0.26 |
| AmB (2.5 μg/ml) | 80.0 ± 3.25* |
| AmB (2.5 μg/ml) plus 100 μmol of caspase-3 inhibitor | 1.0 ± 0.41 |
a
LLC-PK1 were exposed to no AmB, AmB alone, or in combination with caspase-3 inhibitor as indicated.
b
Values are the means ± the standard deviation of data from duplicates of seven independent experiments. ∗, Value differs significantly from that of the untreated control group (no AmB) (P < 0.05).