TABLE 1.
Human FXIII proteins and their affinities to GPVI‐dimer
| Material (abbreviations) | Details | KD (μM) |
|---|---|---|
|
Plasma FXIIIa (FXIIIA2B2) |
Tetrameric, not activated | 0.216 ± 0.067 |
|
FXIII A‐subunitb (rh‐FXIIIA2) |
Recombinant (rh) Not fully activated form |
0.366 ± 0.047 |
|
FXIII A‐subunit (activated)b (rh‐FXIIIAa) |
Recombinant (rh) Thrombin‐activated form of rh‐FXIIIA2 |
0.140 ± 0.011 |
|
FXIII B‐subunitb (rh‐FXIIIB2) |
Recombinant (rh) | Too low to be determined |
The FXIII proteins were obtained from the following suppliers: aFisher Scientific, Leicestershire, UK; and bZedira, Darmstadt, Germany. Binding affinities (KD) were calculated from the binding curves shown in Figure 1B; data are expressed as values of the mean ± SEM (n = 8). The binding affinity of the fully activated form of the FXIII A‐subunit is comparable to GPVI‐dimer’s affinity to collagen type III (0.183 ± 0.026).
Abbreviations: FVIII, factor VIII; GPVI, glycoprotein VI; SEM, standard error of the mean.