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. 2022 Apr 11;9:826729. doi: 10.3389/fcvm.2022.826729

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Copyright © 2022 Marchini, Malchow, Caceres, El Rabih, Hansen, Mwinyella, Spiga, Piepenburg, Horstmann, Olawale, Li, Mitre, Gissler, Bugger, Zirlik, Heidt, Hilgendorf, Stachon, von zur Muehlen, Bode and Wolf.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Study workflow for the development of a novel ELISA to detect anti-ApoB IgG- and IgM- plasma levels. (1) ApoB-derived peptides with a length of 15 amino acids were screened in silico for their binding affinity to MHC-II variants (18). A pool of 30 peptides that showed a high affinity to MHC-II in direct in vitro affinity measurements were used to coat a microplate at 5 μg/mL. (2) A total of 307 plasma samples from the TRAFIC cohort were analyzed by an in-house chemiluminescent ELISA (3) to detect circulating anti-ApoB IgG and IgM auto-antibodies. The figure was generated with schematics from Biorender.com.