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. 2022 Apr 11;10:857066. doi: 10.3389/fcell.2022.857066

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Copyright © 2022 Kwack and Lee.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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A proposed strategy using allogeneic DPSC transplantation for pulp regeneration. 1) Dental pulp tissue is removed from an uninfected orthodontic extraction tooth or wisdom tooth (donor). 2) After isolating and culturing DPSCs from dental pulp, cryopreservation of DPSCs or dental pulp itself is performed. 3) DPSCs are expanded using cryopreserved DPSCs or dental pulp tissues according to the patient’s treatment plan for pulpectomy. 4) Signaling molecules and DPSCs are added to the appropriate scaffold and applied to the disinfected pulp space.